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Methods 252 , 8392 (2001)

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Acknowledgments The authors would like to thank the Scientific Research Project Unit of Gazi University (Ankara, Trkiye) for the project grant provided for this work

This will likely require integrating methods to manipulate specific phosphosites, kinases, or phosphatases with assays examining specific GLUT4 trafficking processes

Nuclei were stained with DAPI, and actin was stained with Alexa-568 phalloidin (Life Technologies, A12380)

Our results show for the first time that secretins can facilitate the translocation of molecules other than proteins or protein fibers, considerably expanding the repertoire of substrates of these multifunctional OM gates