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Further analysis by Western blot showed that rhGPx3 mutant with excess amino acid could be recognized by the monoclonal antibody against the hexahistidine tag, but not in the case of the protein treated with factor Xa (Figure 1a and 1b)

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Green fluorescent protein (GFP) and all of its colorful variants are examples of protein tags that intrinsically fluoresce (Nienhaus & Nienhaus, 2021)