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This approach is consistent with narrative reviews aiming to map emerging evidence, generate hypotheses, and identify research gaps, while acknowledging the increased risk of selection bias inherent to non-systematic methodologies

Organoid preparation and culture To generate gastric cancer organoids, fresh tumor tissues were transported on ice in cold DMEM/F12 (Gibco) with primocin (InvivoGen), then washed in cold PBS, minced into fragments (1 mm), and digested for 1 h at 37 C with collagenase and hyaluronidase

10.1056/NEJMoa2031054 184

Graphical abstract Table 1

PMID 34706925