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doi: 10.1186/s12885-023-11537-3 69 NavarroJObradorECarreteroJPetschenIAviJPerezPet al

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Proteins are involved in the production of enzymatic antioxidants, immunoglobulins, and other structural and functional biomolecules, hence, cannot be replaced with any other molecule (Langer, Citation2009)

finally, combining molecular docking techniques to validate the binding modes of drugs and targets, and further evaluating the efficacy and safety of drugs via in vitro cell experiments and in vivo animal experiments (Gogoi and Saikia, 2022

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Steady-state kinetics analysis Researchers also studied steady-state kinetics to understand substrate binding and structure-activity relationships (Jia et al., 2019)