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Description

Cells were washed with washing buffer [BSA (0.3%) + Triton (0.1%) in 1X PBS] and from this step on, reagents from the DUOLINK PLA kit were used according to the manufacturers instructions (OLINK Bioscience, Uppsala, Sweden)

Yet, because pantothenic acid is widely distributed in nature and deficiency is extremely rare in humans, most information regarding the consequences of deficiency has been gathered from experimental research in animals (reviewed in 4)