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Description

NNAL: Nicotine-derived nitrosamine 1-oxide

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PMID 31233103

This combination of storage and turnover introduces a temporal delay between inadequate intake or absorption and the onset of deficiency

Pauwels, F., Vergauwen, B., Vanrobaeys, F., Devreese, B

Pellets were resuspended in 3 mL of assay buffer (250 mM KCl, 2 mM MgCl 2 , 40 mM HEPES-KOH pH 7.9, 5 mM -mercaptoethanol, 10% (v/v) glycerol, 10 mM imidazole), and 1.25 mL of this resuspension was then used in the pull-down