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The pretreatments of slides of immunofluorescence assay were the same as for IHC assay, including dewaxing, rehydration, retrieval, and 3% H 2 O 2 treatment

As of 17 December 2023, there have been 772 million confirmed cases of COVID-19 worldwide, including 6.9 million deaths, reported to the World Health Organization [2]

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Likewise, caspase-3 (estimated using CaspGLOLO fluorescein) activation was elevated in the utricles treated with gentamicin alone and reduced when the aminoglycoside was given in combination with 50 M melatonin