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Epub 06/01/2016

Penicillamine was also shown to bind Fe 2+ (Leggio et al., 2005)

Moya-Perez A, Perez-Villalba A, Benitez-Paez A, Campillo I, Sanz Y

sgRNA complexes are synthesized by mixing equal volumes of crRNA (2 L, 160 M in nuclease free water) and tracrRNA (2 L, 160 M) and are incubated at 37 C for 30 min before Cas9 (2.7 L, 10 g/L, Alt-R S.p