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Organoid immunofluorescence Organoids were recovered using Cell Recovery Solution (354253, Corning BD) and fixed with 4% paraformaldehyde (PFA) for 20 min at room temperature (1821 C)

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Among cases of severe hepatotoxicity occurring during VPA therapy, survival has been reported mainly in those patients treated with carnitine [6467], and this approach is likely to be biased

The effect of the CPT system on different tissues or organs is complex: the deficiency or over-activation of CPT disrupts the immune homeostasis by causing energy metabolism disorder and inflammatory oxidative damage and therefore contributes to the development of various acute and chronic inflammatory disorders and cancer

Neuroinflammation is primarily caused by astrocyte and microglial activation and proliferation, activation, and translocation of transcription factors such as NF-B, and increased production of cytotoxic cytokines such as TNF- and IL-1 (Afshin-Majd et al