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The frequency of immune cell subsets was assessed by staining 1 x 10 6 nucleated cells with CD45 (KO), CD56 (PC7), CD138 (APC) (both Beckman Coulter), CD14 (PerCP), CD19 (APC-H7), CD3 (V450) and CD16 (PE) (all BD Biosciences)

Dispose of expired or compromised vials per local medical-waste regulations

The Grading of Recommendation, Assessment, Development, and Evaluation (GRADE) tool was used to assess the certainty of the evidence for the two primary biochemical outcomesserum vitamin B12 concentration increase and homocysteine reductionacross all included studies, and separately for randomised controlled trials (RCTs) and observational studies

Frequently Asked Questions 5-Amino-1MQ is used in in-vitro laboratory research to study cellular signaling, metabolic pathways, gene expression regulation, and enzymatic activity

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