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Subjects were initially treated for 6 months of on-demand therapy and then randomized to 12 months of either a standard prophylaxis regimen (20 to 40 IU/kg every 48 hours) or PK-driven prophylaxis regimen (20 to 80 IU/kg every 72 hours)

The medium was removed, and 0.4 mL of MTT solution (Sigma) was added to each well and incubated for another 4 h

Yes, and doing so can enhance your results

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But Vitamin C addresses the underlying conditions that make hyperpigmentation worse

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