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Next, 20 g of the ground sample was placed in a 1 L round-bottomed flask, after which 350 mL distilled water and 1 mL of a 20 g/mL internal standard solution (heptadecane) were added

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100 l of dimethyl sulfoxide (DMSO) was added after discarding the supernatant to dissolve the formazan, and the optical density at 570 nm (OD570nm) was measured

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