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While the site dries, pull the plunger back on your syringe to your desired measurement

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For the quantification of amino acids, 40 L plasma was mixed with 20 L stable-isotope-labeled internal standard (IS) in sulfosalicylic acid to precipitate proteins, followed by vortexing and centrifugation (4000 rpm, 4 C, 20 min)

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For this, pelleted cells were thawed and resuspended in binding buffer (20 mM Tris-HCl pH 7.0, 300 mM NaCl, 2 mM -mercaptoethanol) supplemented with 0.2 mM serine protease inhibitor AEBSF-HCl (Carl Roth GmbH, cat no