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Besides, IH could promote neuronal apoptosis in normal mice and diabetes mice models, the latter had higher apoptosis levels and could be modulated by high mobility group box-1 protein (HMGB1)/toll-like receptor 4 (TLR4) signal [53]

10.1016/0006-2952(87)90130-4 Biochem

In brief, cDNA was generated using M-MLV reverse transcriptase (Thermo Fisher) from RNA samples isolated from the cultures, and then incubated with Fast SYBR Green Master Mix (Applied Biosystems)

Recently, KD feeding was shown to reverse the tactile allodynia produced in a mouse model of metabolic syndrome 64 (strikingly, the KD in this study had almost twice the fat level of the high-fat/moderate carbohydrate diet that induced metabolic syndrome)