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Tapering off trazodone under medical supervision is recommended to minimize withdrawal effects

For cloning, the amplified products were purified using QIAquick PCR Purification kit (Qiagen Inc) and incubated at 72 C for 10 min in a reaction mixture (25 L) containing 200 M of dATP, 1.5 mM MgCl 2 , 0.625 unit of Taq DNA polymerase and 1X buffer, to add A-tail